normal esophageal epithelial cell lines heec  (ATCC)

Journal: Cancer Medicine

Article Title: The GNL3L ‐ MDM2 Interaction Drives Esophageal Squamous Cell Carcinoma Progression

doi: 10.1002/cam4.71146

Figure Lengend Snippet: Expression of GNL3L in esophageal cancer (EC) and the cell line model of GNL3L knockdown. (a) GNL3L expression was analyzed in EC tumor tissues ( n = 182) and normal esophageal tissues ( n = 286) using the GEPIA database. (b) Kaplan–Meier analysis of survival rates between patients with EC and low expression of GNL3L ( n = 138) vs. high expression of GNL3L ( n = 46). In addition to the expression of GNL3L , the survival rate was also related to tumor grade. The patient cohort was obtained from the gene information of TCGA Esophageal cancer datasets downloaded from the UALCAN website. (c) Immunohistochemistry was performed to detect differential expression of GNL3L protein in an esophageal squamous cell carcinoma (ESCC) specimen. (d) qRT‐PCR was used to detect GNL3L expression in ESCC cell lines (TE‐1, KYSE‐410, KYSE‐30, and EC9706) and human normal esophageal epithelial cell line (HEEC). (e) The expression of GNL3L protein in ESCC cell lines and HEEC was assessed using western blotting (WB). (f) Efficiency of sh‐GNL3L for GNL3L in TE‐1 cells by qRT‐PCR. (g) The protein expression of GNL3L in TE‐1 cells. (NC: Negative control). (* p < 0.05, ** p < 0.01).

Article Snippet: ESCC (TE‐1, KYSE‐410, KYSE‐30, EC9706, and ECA‐109) and human normal esophageal epithelial (HEEC) cell lines were obtained from Procell Life Science &Technology Co. Ltd. (Wuhan, China).

Techniques: Expressing, Knockdown, Immunohistochemistry, Quantitative Proteomics, Quantitative RT-PCR, Western Blot, Negative Control

Journal: Journal of Cancer

Article Title: Identification and Characterization of Oxidative Stress and Endoplasmic Reticulum Stress-Related Genes in Esophageal Cancer

doi: 10.7150/jca.104376

Figure Lengend Snippet: TFRC promotes the proliferation, migration, and invasion of ESCA cells. A TFRC expression levels were analyzed in ESCA cells and HEEC using qRT-PCR. B The transfection efficiency of TFRC overexpression in TE1 cells and knockdown in OE33 cells was confirmed by qRT-PCR. C-D Cell proliferation was evaluated in TFRC-overexpressing TE1 cells and TFRC-knockdown OE33 cells using MTS assays (C) and colony formation assays (D). E-F The migration and invasion capabilities of TFRC-overexpressing TE1 cells and TFRC-knockdown OE33 cells were assessed through transwell migration (E) and invasion (F) assays. Data are presented as the mean ± SD from at least three independent experiments. *P < 0.05.

Article Snippet: Human ESCC cell lines (KYSE150 and TE1), an EAC cell line (OE33), and a human normal esophageal epithelial cell line (HEEC) were obtained from the China Center for Type Culture Collection (CCTCC, Wuhan, China).

Techniques: Migration, Expressing, Quantitative RT-PCR, Transfection, Over Expression, Knockdown

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: SAA1 is highly expressed in ESCC tissues and cell lines. A SAA1 mRNA expression in ESCC tissues in TCGA and GEO datasets was analyzed. B SAA1 protein expression in ESCC tissues determined by western blot analysis (n = 16). C SAA1 mRNA and protein expression in HEEC cells and ESCC cell lines was determined by RT‒qPCR. D The cDNA sequencing results of SAA1 in two ESCC cell lines showed that a single nucleotide polymorphism was observed at nucleotide position 209, as indicated by the arrows. **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: A human normal esophageal epithelial cell line HEEC was obtained from ScienCell (CA, USA).

Techniques: Expressing, Western Blot, Sequencing

Journal: Discover. Oncology

Article Title: SAA1 regulated by S1P/S1PR1 promotes the progression of ESCC via β-catenin activation

doi: 10.1007/s12672-024-00923-3

Figure Lengend Snippet: S1P/S1PR1 increases SAA1 expression levels and β-catenin phosphorylation at Ser675 in ESCC cells. A ESCC cells were stimulated with different concentrations of S1P (0, 10, 100, and 1000 nmol/L) for 24 h. SAA1, total β-catenin and pSer675-β-catenin protein levels were detected by western blotting. B ESCC cells were treated with VPC23019 (1000 nmol/L) and then stimulated with S1P (1000 nmol/L) for 24 h. The levels of SAA1, total β-catenin and pSer675-β-catenin were detected by western blotting. C The expression of S1PR1 and S1PR3 proteins in HEEC cells and two ESCC cell lines was detected by western blotting. D The efficiency of S1PR1 overexpression was determined by RT‒qPCR and western blotting. E The efficiency of S1PR1 knockdown was determined by RT‒qPCR and western blot. F The protein levels of SAA1, total β-catenin and pSer675-β-catenin were detected by western blot after S1PR1 overexpression and knockdown in ESCC cells. **p < 0.01, ****p < 0.0001

Article Snippet: A human normal esophageal epithelial cell line HEEC was obtained from ScienCell (CA, USA).

Techniques: Expressing, Phospho-proteomics, Western Blot, Over Expression, Knockdown